scRNA-seq analysis of f8 and vwf expression in endothelial cells isolated from kdrl-egfp transgenic zebrafish larvae. (A) UMAP (uniform manifold approximation and projection) projection scRNA-seq data from endothelial cells showing f8 expression localized specifically to the endocardial cluster, marked by a red circle. (B-C) Colocalization analysis of f8 with endocardial endothelial–specific markers gata5 and has2 confirms endothelial-specific expression of f8. The red circle marks the endocardial cluster. (D) Expression pattern of vwf, demonstrating localization in both the endocardial (red circle) and arterial endothelial (black circle) clusters.