Persistency of MKs and infiltration of T cells in the BM over the course of BMF. (A) Image processing workflow for multi-photon images. (B) MPM imaging of mouse sterna with CD41 APC channel (teal), demonstrating persisting MK signal over the course of BMF. (C) Large CD41⁺ cells coexpress CD42d, split channel image of cells identified as MKs, left to right, CD41 channel, CD42d channel, DAPI channel, merged channel. (D) Individual value plots of MK per image volume over the course of BMF. (E) MPM imaging of DsRed lymphocytes infiltrating into the BM cavity over the course of BMF. D, day; DAPI, 4′,6-diamidino-2-phenylindole; PE, phycoerythrin.