Expression levels of transcripts identified through WGCNA. (A) Mean expression levels of 524 transcripts. Mean expression of all 524 genes for individuals with and without SCD from the single-center cohort (2 columns on left) and multicenter cohort (2 columns on right), each row represents a gene. (B) Individual expression levels of transcripts grouped by center. Variation in gene expression between individuals with SCD, separated by different centers (unique centers labeled A through F, total number of individuals in each center indicated in parentheses). The columns within each center group represent an individual from the respective center (labeled as A through F), each row represents a gene. (C) Annotated heat map of expression levels of pain-related genes. Variation in gene expression displayed of genes significantly associated with clinical pain data in the study cohort and that have been shown to be related to pain in the published literature. Each row represents a gene. The columns within each group (separated by centers A through F) represent an individual from the respective center. Most of the genes (60%) are from the blue module. Note: the individuals in panels B-C are ordered by hierarchical clustering that is done center by center using Genesis. For each center, hierarchical clustering was done based on gene expression level.