Cellular responses to VEN/DEC in AML cell lines. (A) The indicated cell lines were treated with the indicated doses of VEN and/or DEC. PPARG messenger RNA (mRNA) levels were quantified by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and relative expression was normalized to GAPDH expression. (B) PPARG DNA methylation levels in CpG islands at TSS were determined by bisulfite pyrosequencing. The methylation level of untreated cells was set to 100%. (C) Immunoblots showing the amounts of PPARγ protein in each cell line after treatment with the indicated doses of VEN and/or DEC for 3 days. Relative expression of PPARγ was normalized to that of β-actin and the expression level of untreated cells was set to 1.0. (D) The cell lines were treated with the indicated doses of VEN and/or DEC for 3 days. CD36 mRNA levels were quantified by qRT-PCR. Relative expression of CD36 was normalized to GAPDH expression. Data are expressed as the mean ± standard deviation (SD) of 3 independent experiments. Differences between the groups were tested using the 2-tailed Student t test. ∗ vs control, P < .05.