Analysis of cellular bioenergetics in PPARG-OE cells. (A) A Seahorse Bioscience XFe24 Extracellular Flux Analyzer was used to evaluate OCR in control vector vs PPARG-OE cells. MV4;11 and HL60 cells transfected with either the control vector or PPARG-OE construct were treated with VEN ± DEC for 4 hours, after which 5 × 105 cells were added per well. Three technical replicates were plated. During the assay, oligonomycin, FCCP, rotenone, and antimycin A were injected at the times indicated by the arrows. SRC percent was calculated as follows: (maximal OCR/basal OCR × 100). (B) Endogenous FAO was measured with or without etomoxir after nutrient starvation. The maximal OCRs are shown. Data are expressed as the mean ± SD of 3 independent experiments. Differences between the groups were tested using the 2-tailed Student t test. ∗P < .05.
