BCLAF1 associates with chromatin in hematopoietic progenitor cells. (A) Heat map of signal intensity for BCLAF1 peaks identified by native CUT&RUN in Vav-Cre:Bclaf1^f/f and Bclaf1^f/f E17.5 fetal HSCs. Vav-Cre:Bclaf1^f/f HSCs included as negative control. (B) Annotation of genomic location for BCLAF1 peaks identified in Bclaf1^f/f HSCs in panel A. (C) Heat map of signal intensity for BCLAF1 peaks identified by native and fixed CUT&RUN in adult BM HSPCs (Lin^-Sca⁺Kit⁺) from adult Bclaf1^f/f mice. (D) Annotation of genomic location for BCLAF1 peaks identified in native and fixed CUT&RUN in panel C. (E) Venn diagram comparing BCLAF1 peaks identified by native CUT&RUN in fetal liver HSCs in panel A vs native adult BM HPSCs in panel C. (F) Venn diagram comparing BCLAF1 peaks identified by native vs fixed CUT&RUN in the BM HSPCs in panel C. (G) Representative tracks at indicated regions for BCLAF1 peaks identified by fixed CUT&RUN in adult BM HSPCs in panel C. (H) Top 2 results from Hypergeometric Optimization of Motif EnRichment (HOMER)⁷⁶ known motif analysis of BCLAF1 peaks identified in Bclaf1^f/f HSCs in panel A. NA, not available; Refseq, reference sequence; UTR, untranslated region.