Figure 7.
CSPα−/− mice have reduced bone marrow megakaryocyte numbers and increased collagen I deposition. (A) Representative images of femur bone marrow from CSPα+/+ and CSPα−/− mice (aged 5-6 weeks). Collagen I (yellow), CD105 (purple), DAPI (blue), and CD41 (cyan). Scale bar, 20 μm. (B) Representative scan images of the femur from CSPα+/+ and CSPα−/− mice stained for CD41 (megakaryocyte). (C) Reticulin staining of marrow from CSPα−/− mice. Scale bar, 50 μm. Supplemental Figure 8 for WT marrow and fibrotic liver controls. (D) Quantification of megakaryocytes per area, megakaryocyte size, and collagen I intensity in the femurs of CSPα+/+, CSPα+/−, and CSPα−/− mice. Bones from CSPα+/+ (n = 4; male = 3, and female = 1), CSPα+/− (n = 3; male = 2, and female = 1), and CSPα−/− (n = 4; male = 2, and female = 2) mice were evaluated. Megakaryocytes per area and megakaryocyte size were evaluated by examining the number and size of CD41+ cells. Quantification of collagen I fluorescence intensity was measured within each image. Statistical analyses were performed using individual values and the Kruskal-Wallis multiple comparison test and corrected using the Dunn multiple comparison test. The P values are indicated. Scale bar, 20 μm.

CSPα−/− mice have reduced bone marrow megakaryocyte numbers and increased collagen I deposition. (A) Representative images of femur bone marrow from CSPα+/+ and CSPα−/− mice (aged 5-6 weeks). Collagen I (yellow), CD105 (purple), DAPI (blue), and CD41 (cyan). Scale bar, 20 μm. (B) Representative scan images of the femur from CSPα+/+ and CSPα−/− mice stained for CD41 (megakaryocyte). (C) Reticulin staining of marrow from CSPα−/− mice. Scale bar, 50 μm. Supplemental Figure 8 for WT marrow and fibrotic liver controls. (D) Quantification of megakaryocytes per area, megakaryocyte size, and collagen I intensity in the femurs of CSPα+/+, CSPα+/−, and CSPα−/− mice. Bones from CSPα+/+ (n = 4; male = 3, and female = 1), CSPα+/− (n = 3; male = 2, and female = 1), and CSPα−/− (n = 4; male = 2, and female = 2) mice were evaluated. Megakaryocytes per area and megakaryocyte size were evaluated by examining the number and size of CD41+ cells. Quantification of collagen I fluorescence intensity was measured within each image. Statistical analyses were performed using individual values and the Kruskal-Wallis multiple comparison test and corrected using the Dunn multiple comparison test. The P values are indicated. Scale bar, 20 μm.

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