Figure 2.
Distinct DNA methylation at human LIN28A CpG island 2 in let-7 low and let-7 high imMKCLs. (A) A schematic representation of CpG islands in the human LIN28A gene. Two CpG islands sequences were identified using the MethPrimer platform. Bisulfite PCR was performed, and the methylation status of each CpG island was evaluated. (B) The methylation status of individual CpG dinucleotides in human LIN28A CpG islands was analyzed by bisulfite sequencing in let-7high and let-7low cells. Results are shown by methylated (closed) or unmethylated (open) circles. (C) Individual and total methylation rates of 2 CpG islands are shown. Fisher exact tests were used to assess statistical significance by QUMA software. ∗P < .05; ∗∗P < .01. ns, not significant.

Distinct DNA methylation at human LIN28A CpG island 2 in let-7 low and let-7 high imMKCLs. (A) A schematic representation of CpG islands in the human LIN28A gene. Two CpG islands sequences were identified using the MethPrimer platform. Bisulfite PCR was performed, and the methylation status of each CpG island was evaluated. (B) The methylation status of individual CpG dinucleotides in human LIN28A CpG islands was analyzed by bisulfite sequencing in let-7high and let-7low cells. Results are shown by methylated (closed) or unmethylated (open) circles. (C) Individual and total methylation rates of 2 CpG islands are shown. Fisher exact tests were used to assess statistical significance by QUMA software. ∗P < .05; ∗∗P < .01. ns, not significant.

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