Mechanical properties of nonsickled, irreversibly sickled, and RBCs with detectable Hb polymer. (A) Representative images of nonsickled RBCs and cells classified as sickled by the CNN that have been separated into ISCs and RBCs containing polymerized Hb based on the oxygen tension and cell saturation. (B) Deformation parameter of the 3 cell types for 10 HbSS blood samples and 2 HbAA blood samples. (C) Mean shear surface modulus of the 10 HbSS blood samples and HbAA blood samples calculated from the corresponding deformation parameter. A 1-way analysis of variance was used to identify significant differences, and a Dunnett test was used to compare the subpopulations.