Reduced abundance of ribosomal proteins in Npm1^cA/+ hematopoietic progenitors. (A) Workflow for global quantitative proteomic study of lineage-negative (lin^–) bone marrow–derived hematopoietic progenitors using TMT. Preleukemic Npm1^cA/+ (n = 7) vs Npm1^+/+ (n = 5) murine hematopoietic progenitors were collected 8 weeks after Mx1-Cre induction with pIpC. (B) Volcano plot revealing relative protein abundances in preleukemic Npm1^cA/+ vs Npm1^+/+ lin^– progenitors. (C-F) Relative abundance of selected ribosome-related proteins in Npm1^cA/+ vs Npm1^+/+ lin^– progenitors. (G) Overlap between messenger RNAs (RNA-seq) and proteins (LC-MS) that were significantly over- or under-expressed in preleukemic Npm1^cA/+ vs Npm1^+/+ lin^– cells. Panel B, unpaired t test with multiple-hypothesis–testing correction (FDR; Benjamini-Hochberg). (C-F) Unpaired t test with multiple-hypothesis–testing correction (FDR; Benjamini-Hochberg). ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ .001; ∗∗∗∗P ≤ .0001. Data represent n = 7 (Npm1^cA/+) or n = 5 (Npm1^+/+) biological replicates (mean ± SD). FDR, false discovery rate; LC-MS TMT, liquid chromatography mass spectrometry, tandem mass tagging; pIpC, polyinosinic:polycytidylic acid. Panels A and G were created with BioRender.com. Vassiliou, G. (2025) https://BioRender.com/9tt8kww.