In vitro platelet mixing study. Platelet concentrate obtained from donor 1 was mixed with platelet concentrate of donor 2 with a predefined ratio (1:1, 1:5, 1:10, 1:20, and 1:50). Each mixed platelet concentrate was subjected to the NGS-based platelet tracking method, which yielded the ratio of the platelet of donor 1. The ratio of donor 1 platelets at each marker position was plotted (red dots) and used to calculate linearity. The expected and measured ratios of donor 1 platelet are shown.