Figure 2.
Lipidomic analysis revealed changes in lipid profiles of ACC1 pKO platelets. Lipid extraction was performed on washed murine platelets from 6 samples for 1 genotype, each sample consisting of platelets pooled from 2 or 3 mice of the same genotype. (A) Platelet lipidome overview of ACC1 pKO, and ACC1flx/flx, and GPIbα-Cre+/− controls by principle component analysis. The colors and symbols distinguish the 3 genotypes. The first and second dimensions with their associated percentage of explained variance are displayed on the x-axes and y-axes, respectively. (B-F) Lipidomic analysis comparing ACC1 pKO and ACC1flx/flx platelets. (B) Box and scatter plot representations of the modulation (log2FC) of 446 identified lipid species categorized into 5 (sub)classes, each represented by a distinct color (red, FA; orange, GL; yellow, PL; green, SL; and blue, ST). Lipid species are represented as black or gray dots based on their statistical significance (black dots representing a significant adjusted P value, FDR ≤ 0.05; whereas gray dots correspond to FDR ≥ 0.05). Lipids above the horizontal dotted line are upregulated, whereas lipids below are downregulated. Log FCs and FDR were calculated from the multivariate regression model. (C-E) Dot plots displaying the length and unsaturation levels of the 2 fatty acyl chains from PC (C), PE (D), and PEP (E). Color bars indicate the logFC from red (upregulation) to blue (downregulation). The adjusted P value is represented by the circle diameter (the largest circle corresponds to FDR ≤ 0.05). (F) Forest plot of AA-containing PC, PE, and PEP comparing ACC1flx/flx and ACC1 pKO mice. Data are presented as logFC (squares) relative to ACC1flx/flx platelets. (G) Radar plot showing the relative intensity of PC, PE, and PEP containing the 20:4 fatty acyl chains. The dark gray area corresponds to ACC1flx/flx mice, whereas the orange area corresponds to ACC1 pKO mice. CE, cholesterol ester; DG, diacylglycerol; FA, fatty acid; FC, fold change; GL, glycerolipid; HCER, hydroxyceramide; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PL, phospholipid; SL, sphingolipid; SM, sphingomyelin; ST, sterol; TG, triacylglycerol.

Lipidomic analysis revealed changes in lipid profiles of ACC1 pKO platelets. Lipid extraction was performed on washed murine platelets from 6 samples for 1 genotype, each sample consisting of platelets pooled from 2 or 3 mice of the same genotype. (A) Platelet lipidome overview of ACC1 pKO, and ACC1flx/flx, and GPIbα-Cre+/− controls by principle component analysis. The colors and symbols distinguish the 3 genotypes. The first and second dimensions with their associated percentage of explained variance are displayed on the x-axes and y-axes, respectively. (B-F) Lipidomic analysis comparing ACC1 pKO and ACC1flx/flx platelets. (B) Box and scatter plot representations of the modulation (log2FC) of 446 identified lipid species categorized into 5 (sub)classes, each represented by a distinct color (red, FA; orange, GL; yellow, PL; green, SL; and blue, ST). Lipid species are represented as black or gray dots based on their statistical significance (black dots representing a significant adjusted P value, FDR ≤ 0.05; whereas gray dots correspond to FDR ≥ 0.05). Lipids above the horizontal dotted line are upregulated, whereas lipids below are downregulated. Log FCs and FDR were calculated from the multivariate regression model. (C-E) Dot plots displaying the length and unsaturation levels of the 2 fatty acyl chains from PC (C), PE (D), and PEP (E). Color bars indicate the logFC from red (upregulation) to blue (downregulation). The adjusted P value is represented by the circle diameter (the largest circle corresponds to FDR ≤ 0.05). (F) Forest plot of AA-containing PC, PE, and PEP comparing ACC1flx/flx and ACC1 pKO mice. Data are presented as logFC (squares) relative to ACC1flx/flx platelets. (G) Radar plot showing the relative intensity of PC, PE, and PEP containing the 20:4 fatty acyl chains. The dark gray area corresponds to ACC1flx/flx mice, whereas the orange area corresponds to ACC1 pKO mice. CE, cholesterol ester; DG, diacylglycerol; FA, fatty acid; FC, fold change; GL, glycerolipid; HCER, hydroxyceramide; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PL, phospholipid; SL, sphingolipid; SM, sphingomyelin; ST, sterol; TG, triacylglycerol.

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