Platelet-specific ACC1 deletion affects platelet spreading and actin cytoskeleton organization upon CRP stimulation. (A-E) Washed murine platelets were stimulated with or without CRP 0.3 μg/mL for 30 minutes. Platelets were stained with phalloidin-fluorescein isothiocyanate for 45 minutes at room temperature. (A) Panel of representative pictures. Scale bar, 5 μm. (B-C) Quantification of the mean surface area covered by (B) unstimulated or (C) CRP-stimulated platelets independently of the platelet morphology. Data are expressed as mean ± SEM (n = 4). #P ≤ .05 relative to ACC1^flx/flx mice. Data underwent 1-way ANOVA. (D-E) Proportion of the surface area covered by (D) unstimulated or (E) CRP-stimulated platelets, being inactivated (discoid), extending filopodia, forming lamellipodia or fully spread after activation. Data are expressed as means ± SEM (n = 4). #P ≤ .05 relative to ACC1^flx/flx mice. $P ≤ .05 relative to GPIb-Cre^+/− mice. Data underwent 2-way ANOVA.