ACC1 pKO mice exhibited reduced arterial thrombosis while maintaining normal hemostasis. (A) Tail bleeding times of ACC1^flx/flx, GPIb-Cre^+/−, and ACC1 pKO mice in saline solution preheated at 37°C. Results are expressed as mean ± SEM (n ≥ 23). Data were analyzed via 1-way ANOVA. (B-D) Mice were subjected to thrombosis induced in vivo by FeCl₃ application to the carotid artery (10% of FeCl₃ during 5 minutes). Thrombus formation was visualized using intravital microscopy analyzing exogenous platelet accumulation stained with carboxyfluorescein succinimidyl ester. (B-C) Thrombus growth area quantification over time. Data are expressed as mean ± SEM (n ≥ 6). #P ≤.05 relative to ACC1^flx/flx mice. Data were analyzed using unpaired t test comparing area under the curve. (D) Representative pictures of thrombus formation over time (1, 5, 9, 15, and 19 minutes) after FeCl₃ injury.