![Genetic loss of plasminogen does not increase venous thrombus formation or change cellular or fibrin(ogen) deposition. (A) PA curves in PPP from Plg+/+, Plg+/−, Plg–/– mice; representative of N = 5 to 9 mice per genotype. (B-C) The mass of venous thrombi harvested from Plg+/+, Plg+/−, Plg–/– mice 24 or 6 hours after IVC ligation. (D) Representative images of 6-hour thrombi probed to identify nuclei/DNA (DAPI [4′,6-diamidino-2-phenylindole], blue), platelets (anti-CD41, red), or fibrin(ogen) (polyclonal antibody, green). Scale bars indicate 100 μm. Methods for acquiring and processing images are provided in the supplemental Materials. (E) Quantification of nuclei, platelets, and fibrin(ogen) in 6-hour thrombi. Graphs show mean ± standard deviation or median ± interquartile range, as appropriate; each dot indicates a separate mouse. Groups were compared by ordinary 1-way analysis of variance with Šidák multiple comparisons test or Kruskal-Wallis test with Dunn multiple comparisons test for normally and nonnormally distributed data, as appropriate; no groups were significantly different.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/146/11/10.1182_blood.2025028680/2/blood_bld-2025-028680-gr1.jpeg?Expires=1760662743&Signature=FEc5mGJ0vbqTiWHczutE7tp9DUrHk01-r~wKclOP7dF1qJ-sLqZJRpPoaK~G0n0QZnEIEMTUI4y-gCDRkz1uMFXA4JEWkk0nwrs803Xvb8P9Tw~KhjZbWCuTNsKSjac587DPtbww3-lhpKGdC8e6xjVc7TRKb2EQJ~7VPjV418r0Pc~8ZM6it2nL1f6HALePNxU3zxuzqBo47Rd4LKCmK15ap70eVlks86wCBv9g1O8lfxz3oNbNanz-b49LtuNwESfzgQgdVbtwF7cFW6L-bNOMpE2nnV6XO3YPWKygSYMjzuWF-l9BGClyPLtlptWXF5HBmIRV0Qov2lUSKgz1iw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Genetic loss of plasminogen does not increase venous thrombus formation or change cellular or fibrin(ogen) deposition. (A) PA curves in PPP from Plg+/+, Plg+/−, Plg–/– mice; representative of N = 5 to 9 mice per genotype. (B-C) The mass of venous thrombi harvested from Plg+/+, Plg+/−, Plg–/– mice 24 or 6 hours after IVC ligation. (D) Representative images of 6-hour thrombi probed to identify nuclei/DNA (DAPI [4′,6-diamidino-2-phenylindole], blue), platelets (anti-CD41, red), or fibrin(ogen) (polyclonal antibody, green). Scale bars indicate 100 μm. Methods for acquiring and processing images are provided in the supplemental Materials. (E) Quantification of nuclei, platelets, and fibrin(ogen) in 6-hour thrombi. Graphs show mean ± standard deviation or median ± interquartile range, as appropriate; each dot indicates a separate mouse. Groups were compared by ordinary 1-way analysis of variance with Šidák multiple comparisons test or Kruskal-Wallis test with Dunn multiple comparisons test for normally and nonnormally distributed data, as appropriate; no groups were significantly different.
