In vitro traumatization induces transcriptional changes associated with endothelial dysfunction in healthy ECFCs. Bulk RNA-seq was performed on healthy ECFCs cultured under untrau or trau conditions for 2 and 24 hours, representing the early and late effects of trauma, respectively. (A) Volcano plot showing differentially expressed genes (DEGs) in 2-hour trau ECFCs compared to 2-hour untrau ECFCs, with –log₁₀P and log₂ fold change thresholds set to 1.3 (P = .05) and 1 (fold change = 2), respectively. Dot plots showing the top 20 significantly upregulated (B) and downregulated (C) GO biological processes in 2-hour trau ECFCs. (D) Volcano plot showing DEGs in 24-hour trau ECFCs compared to 24-hour untrau ECFCs with –log₁₀P and log₂ fold change thresholds set to 1.3 and 1, respectively. Dot plots showing the top 20 significantly upregulated (E) and downregulated (F) GO biological processes in 24-hour trau ECFCs. GPI, glycosylphosphatidylinositol; NADH, reduced nicotinamide adenine dinucleotide; ncRNA, noncoding RNA; rRNA, ribosomal RNA; tRNA, transfer RNA.