In vitro traumatization induces changes in the hemostatic transcriptome of healthy ECFCs. Bulk RNA-seq analysis revealed differential expression of key endothelial hemostatic genes between ECFCs, cultured under untrau and trau conditions for 2 hours or 24 hours. (A-B) Log₂ transformed CPM values for transcripts of fibrinolysis-associated (A) and coagulation-associated (B) genes in 2-hour untrau or trau ECFCs. (C-D) Log₂ transformed CPM values for transcripts of fibrinolysis-associated (C) and coagulation-associated (D) genes in 24-hour untrau or trau ECFCs. Statistical analysis was performed using the Wilcoxon test for paired comparisons in panels A-D, with P value <.05 considered statistically significant. CPM, counts per million.