In vitro traumatization of healthy ECFCs induces global hemostatic changes in healthy plasma resembling those observed in plasma from trauma patients. (A) TG in control (biological replicate, N = 10; technical replicate, n = 2) or trauma plasma (biological replicate, N = 10; technical replicate, n = 2) over time in the presence of healthy ECFCs cultured under untrau or trau conditions for 2 hours (i), with derived TG parameters: lag times (ii), ETP (iii), peak thrombin (iv), and times to peak thrombin (v). (B) Clot formation and lysis in control (biological replicate, N = 5; technical replicate, n = 3) or trauma plasma (biological replicate, N = 10; technical replicate, n = 3) over time in the presence of healthy ECFCs cultured under untrau or trau conditions for 2 hours (i), with corresponding CLT₅₀ (ii). ECFCs were traumatized for 2 hours to model the early phase of endothelial dysfunction, consistent with the clinical context of the trauma plasma samples, which were collected shortly after hospital admission. Data are presented as median with interquartile range. Statistical analysis was performed using the Wilcoxon test for paired comparisons and the Mann-Whitney test for unpaired comparisons in panels Aii-v,Bii; with P value <.05 considered statistically significant.