![Decreased expression of surface markers and biophysical characteristics of nascent reticulocytes. (A) Flow cytometry of nascent reticulocytes (red histograms) vs mature red blood cells (gray histograms) for the different surface markers indicated. (B) Fluorescence-imaged micropipette deformation experiments performed on GPC (phycoerythrin-conjugated), CD59 (fluorescein isothiocyanate [FITC]-conjugated), CD44 (FITC-conjugated), and CD71 (FITC-conjugated). CD44 and CD71 were dual-stained with band 3 (phycoerythrin-conjugated).](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodrci/1/2/10.1016_j.brci.2025.100012/4/brci_rci-2025-000156-gr2.jpeg?Expires=1762263899&Signature=pcOnp~zSyj2yaFnEXKHQDYGSQEx1qLO02Y9v6nQKElvl0ys90pW2-JaNfzDK90GzoprFjwc8BUJq0NjrSmNHBewYTvP1FZ69K9aGaikewYZUk1qiBz3Khg5V-UBQXOvEiuA5duPBz13vUZUw~NyXvzsMJIvekDjDrlGSslVRU7LhTaQsTUcPi9vrkXUrZAwns~ymG3gRUHfk41qDJyB6hbo7oXtrlHm0cr-TJNrXFOtyX~vQQ374n3xcELxsyVfnsMvSv-073ragbowb43kqlG5KBz8w~65X6v3hjltFl9KSoRzhnYX1M47GBewuQ3ci4qP1g7sVH1ZdJlWSJdLrtg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 2.
Decreased expression of surface markers and biophysical characteristics of nascent reticulocytes. (A) Flow cytometry of nascent reticulocytes (red histograms) vs mature red blood cells (gray histograms) for the different surface markers indicated. (B) Fluorescence-imaged micropipette deformation experiments performed on GPC (phycoerythrin-conjugated), CD59 (fluorescein isothiocyanate [FITC]-conjugated), CD44 (FITC-conjugated), and CD71 (FITC-conjugated). CD44 and CD71 were dual-stained with band 3 (phycoerythrin-conjugated).
