M1 protein affects fibrin clot architecture. (A) Representative LSCM images of plasma fibrin clots in the presence of rM1 protein (0-150 μg/mL) (n = 3). Clots were analyzed using a Leica TCS SP8X MP OPO spectral confocal microscope and images using 63× water immersion objective (pin hole: 1.0 airy, 16-bit resolution). (B) Fibers (n = 20) were selected at random per condition and quantified in terms of their diameters using ImageJ. (C) Fibrin fiber density was calculated from LSCM images by applying a grid overlay and counting the number of fibers crossing the lines (n = 3). Error bars represent standard error of the mean. (∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001, 1-way ANOVA with Dunnett post hoc test).
Figure 4.

M1 protein affects fibrin clot architecture. (A) Representative LSCM images of plasma fibrin clots in the presence of rM1 protein (0-150 μg/mL) (n = 3). Clots were analyzed using a Leica TCS SP8X MP OPO spectral confocal microscope and images using 63× water immersion objective (pin hole: 1.0 airy, 16-bit resolution). (B) Fibers (n = 20) were selected at random per condition and quantified in terms of their diameters using ImageJ. (C) Fibrin fiber density was calculated from LSCM images by applying a grid overlay and counting the number of fibers crossing the lines (n = 3). Error bars represent standard error of the mean. (∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001, 1-way ANOVA with Dunnett post hoc test).

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