Kinetics of thrombus formation at a venous shear rate. (A) Laser pulse to the mesenteric vein endothelium induces an endothelial injury of ∼20 μm. A representative image is shown. (B) Kinetics of platelet thrombus over time after laser pulse of the mouse mesenteric vein endothelium. Mean platelet surface area coverage over time (line) and standard error of the mean (shaded line) from n = 7 thrombi from n = 3 C57BL/6 mice. (C) Platelet AUC and end point fibrin volume from n = 28 thrombi produced by laser injury of mesenteric veins in n = 9 C57BL/6 mice. Data are presented as individual values (circles), mean (line), and CV. (D) Endo-chip laser-induced thrombus formation model. Blood was incubated with annexin V, anti-CD41 antibody, and antifibrin antibody, recalcified with CaCl2 (10 mM), and then immediately perfused through the Endo-chip at a venous shear rate of 100 s–1, followed by a laser pulse to the endothelium. Representative images of annexin V (red), platelets (CD41, green), and fibrin (magenta) at 0, 1, and 10 minutes from laser injury are shown. (E) Kinetics of annexin V (red), platelet (green), and fibrin (red) surface area coverage over time in the Endo-chip after laser injury. Mean surface coverage over time (line) and standard error of the mean (shaded line) from n = 3 different blood donors. (F) Platelet and fibrin AUC from 14 different donors over different days. Data are presented as individual values (circles), mean (line), and CV. AUC, area under the curve; CV, coefficient of variation.
