TF localizes to the endothelial injury site, and its inhibition reduces thrombus formation in the laser-injury Endo-chip. (A) Blood was labeled with annexin V-AF647, anti-TF antibody 5G9-Atto488 (2 μg/mL, detection dose), and anti-fibrin-594 antibody, recalcified with CaCl₂ (10 mM), and then perfused through the Endo-chip at 100 s^–1 followed by laser injury to the endothelial layer. Representative images of annexin V (red), TF (green), fibrin (magenta) at 0, 1, and 10 minutes after laser injury. (B) Blood was incubated with isotype control or inhibitory anti-TF antibody 5G9 (50 μg/mL, inhibitory dose) and then labeled with anti-CD41 antibody and antifibrin antibody, recalcified, and immediately perfused through the Endo-chip at 100 s^–1 prior to laser injury. Representative images of platelet (CD41, green) and fibrin (magenta) accumulation at the injury site at 10 minutes. (C) Kinetics of platelet (left panel) and fibrin (right panel) surface area coverage over time in response to isotype control or 5G9. (D) Platelet (upper panel) and fibrin (lower panel) AUC after treatment of blood with isotype control or inhibitory anti-TF antibody 5G9. Mean ± SD, n = 6 independent experiments, paired t test. AUC, area under the curve.