Figure 2.
![High-throughput subcellular spatial transcriptomics of human BM. Xenium data of BM trephines from controls (Ctrl-1 to Ctrl-4) and patients with MGUS (MGUS-1 and MGUS-2), SM (SM-1 to SM-5), and MM (MM-1 to MM-10) using a Xenium Prime 5K human pan-tissue and pathways panel. UMAP visualization (A) and proportion (B) of Xenium cells annotated using published scRNA-seq data.19 (C) CD138 immunohistochemistry and spatial plots of annotated Xenium cells of SM-1 and MM-3 samples (scale bars, 1 mm [overview] and 10 μm [magnified areas]). Ba, basophil; Eo, eosinophil; Ma, mast cell; Osteo, osteo-lineage cells; VSMC, vascular smooth muscle cell.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/146/15/10.1182_blood.2025028896/2/blood_bld-2025-028896-gr2.jpeg?Expires=1767031264&Signature=dbrQelKJeMDmX2dlwnfKSX4miB9GmlgxZ7p699jOO0pfdCH2KAHCBX-OlPjif-aCcDFiNYwJB0LocVv1rTAMW40whbZmrx994PSrjEUeMgCVghE~~ua-IbamDyLqRfaZIrIHPEzJrsyL2~0g4Hh~fvwKTXDLxM0s~0Kz86h-nKqboqdeGR0INAeIDSbrEZ6qGE5gagl~itteMeGsgW9RSdtT4KqkAmuK8M80GCcOGMANeoSRUYx7xbEjDSABj~kvSEcPe4-zNkB8n7VQxBnPZACEldoX1fXcX5Rs1~6KEKUWwUGIMWqbR3iiJiVc5hwvOddA9N2w1OT~gIE8JDqjJg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
High-throughput subcellular spatial transcriptomics of human BM. Xenium data of BM trephines from controls (Ctrl-1 to Ctrl-4) and patients with MGUS (MGUS-1 and MGUS-2), SM (SM-1 to SM-5), and MM (MM-1 to MM-10) using a Xenium Prime 5K human pan-tissue and pathways panel. UMAP visualization (A) and proportion (B) of Xenium cells annotated using published scRNA-seq data.19 (C) CD138 immunohistochemistry and spatial plots of annotated Xenium cells of SM-1 and MM-3 samples (scale bars, 1 mm [overview] and 10 μm [magnified areas]). Ba, basophil; Eo, eosinophil; Ma, mast cell; Osteo, osteo-lineage cells; VSMC, vascular smooth muscle cell.
