Spatial neighborhood analysis by scider. (A) UMAP and spatial plots of neighborhoods for representative control (Ctrl-3), MGUS (MGUS-1 and MGUS-2), SM (SM-1 and SM-5), and all MM (MM-1 to MM-10) samples. Colors represent transcriptionally distinct neighborhoods. These neighborhoods are deconvoluted with cell labels derived from published scRNA-seq data to generate density heat map for major BM cell types.¹⁹ The heat map shows the expected number of cells per hexagonal bin (scale bar, 1 mm). (B) Proportion of annotated cells in distinct neighborhoods for samples in panel A. Ba, basophil; Eo, eosinophil; Ma, mast cell; Osteo, osteo-lineage cells; VSMC, vascular smooth muscle cells.