High dimensional analysis of induced, aberrant Ras signals in the mature BM compartment. (A) A balance of dormant and cycling HSCs. (B) pIpC injection of the indicated genetic mouse models induces the desired Ras pathway lesions. (C-E) UMAP representation of cell composition in wild-type BM, RoLoRiG/Mx1CRE total BM, and KRAS^G12D/Mx1CRE total BM. (D) Strategy of using single-cell resolution CyTOF to investigate stem and progenitor cells. Frequencies of cell types are calculated from wild-type mice, based on our CyTOF data. (E) Total BM cell counts in the 20 individual mice. ∗P < .05. (F) CD11b⁺ cells as a percentage of total BM cells for the indicated experimental groups. ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. Other comparisons were not significant. (G) PhenoGraph analysis revealing 23 CD11b⁺ clusters in BM of wild-type mice, compilation of n = 4 mice. Clusters 10 and 11 were identified by PhenoGraph, but their data points were negligible, and cluster 5 was undefined (supplemental Table 2). (H) Relative abundance of the 23 clusters, visualizing overall changes in the CD11b⁺ compartment. Note that cluster 1, indicated by the arrow, is defined by Ly6G⁺, CD16/32⁺, CD43⁺, Ly6C⁺, CD48^+med, CD11b⁺, CD44⁺, indicative of a mature neutrophil population. IP, intraperitoneal; MDSCs, myeloid-derived suppressor cells; NK, natural killer; pDCs, plasmacytoid dendritic cells.