Distinct Ras pathway lesions drive opposing immature BM compartment features. (A) PhenoGraph analysis of Lin^– cells reveal 20 cell clusters in BM. (B-D) PhenoGraph analysis of Lin^– compartment for wild-type BM, RoLoRiG/Mx1CRE total BM, and KRAS^G12D/Mx1CRE total BM. UMAPs are compilations of 4 mice at the indicated time after pIpC injection. (E) Relative abundance of the 20 assigned, Lin^– cell clusters in the BM. Note the frequencies of LSK cells (cluster 4) and CMP (cluster 17). (F) Color-coded visualization of log2 fold changes in cell cluster frequencies of the Lin^– compartment in RoLoRiG/Mx1CRE and KRAS^G12D/Mx1CRE BM, compared with wild-type. (G-H) Frequencies of CMP and LSK stem cells as a percentage of Lin^– BM cells. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. Other comparisons were not significant. (I) Summary of main points for analysis of Lin^– compartment, with the numbers of arrows indicating the magnitude of the alteration. ILC, innate lymphoid cells; MEP, megakaryocyte-erythroid progenitor; pDC, plasmacytoid dendritic cell.