High-resolution analysis of HSC compartments of wild-type, RoLoRiG/Mx1CRE, and KRAS^G12D/Mx1CRE mice. (A) Schematic of HSC and MPP1 to MPP4 annotation,³⁸ applied in this study. (B) PhenoGraph analysis of the wild-type LSK compartment reveals 10 clusters: HSC, MPP1 to MPP4, CLP-like, MPP3-like, MPP4-like, and 2 undefined populations. Compilation of 4 mice. (C) As in panel B, but for KRAS^G12D/Mx1CRE, 2 months after pIpC injection. (D) As in panel B, but for RoLoRiG/Mx1CRE mice. (E) Relative abundance of the 10 clusters in the LSK compartment. (F-I) Frequencies of MPP2, MPP3, MPP4, and HSC cells as a percentage of LSK BM cells for the indicated experimental groups. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. Other comparisons were not significant. CLP, common lymphoid progenitor.