Influence of high-protein diet in a syngeneic colon cancer tumor model. Age-matched C57BL/6 female mice were used as in Figure 1. (A) Averages of tumor volumes per group are shown. Error bars represent SEM. (B) Averages of normalized clot weights are shown. Two-factor analysis of variance (ANOVA; P = .0002). (C) Paraffin-embedded sections of IVC were stained using anti-TF and anti-CD31 antibodies. Alexa Fluor secondary antibodies and DAPI were used. Images are representative of IVCs from 5 mice in each group (scale bar, 100 μm). (D) ID was normalized to the surface area of the region of interest measured in microns by using ImageJ. Averages of ID normalized to surface area are shown. Error bars represent SEM. Two-factor ANOVA test (P = .0003). (E) IVCs were stained by using anti–PAI-1 and anti-CD31 antibodies. Alexa Fluor secondary antibodies and DAPI were used. Representative images from 5 mice in each group (scale bar, 100 μm). (F) PAI-1 expression was quantified as previously described. Averages of ID normalized to the surface area are shown. Error bars represent SEM. Two-factor ANOVA test (P = .0002; ∗∗P = .0255). ^#P < 0.05, ∗P < 0.05.