The expressions of procoagulant and anticoagulant proteins are altered in the IVC of mice with colon cancer tumors and on a high Trp diet. Age-matched C57BL/6 female mice were used as in Figure 1. (A) Representative images of paraffin-embedded sections of IVC from mice with MC38 tumor exposed to a normal, zero, or high Trp diets (n = 10 female mice per group). IVC tissues were stained with indicated antibodies. DAPI was used for nuclear stain (scale bar, 100 μm). (B) Averages of normalized ID of TF normalized to surface area in each of the examined sections. Three images per IVC were analyzed in each mouse. Two-factor ANOVA test (P < .0001). (C) Representative images of tissue section of IVC stained with anti–PAI-1 and anti-CD31 antibodies (scale bar, 100 μm). (D) Averages of normalized ID of PAI-1 normalized to surface area in each of the examined section. ANOVA (P < .0001). (E) Representative images of paraffin-embedded sections of IVC from female mice with MC38 tumor exposed to a normal, zero, or high Trp diets, stained with TM and CD31 (n = 10 mice per group). DAPI was used for nuclear stain (scale bar, 100 μm). (F) Averages of normalized ID of TM expression in endothelial cells normalized to surface area in each of the examined sections. ANOVA (P < .0001). (G) Representative images of paraffin-embedded sections of IVC from mice with MC38 tumor exposed to a normal, zero, or high Trp diets, stained with VWF and CD31 (scale bar, 100 μm). (H) Average normalized ID VWF expression in IVC was measured using a region of interest marked corresponding to the endothelial cells. ANOVA (P < .0001; ∗∗∗P = .0002 [zero vs normal Trp]; P = .0585 [not significant; high vs normal Trp]; ^###P = .002 [high vs zero Trp]).