IDO1 inhibition attenuates thrombosis in a mouse cancer model under different Trp diets. (A) IDO activity measured in the sera of mice with and without MC38 tumor. A group of age-matched 8- to 12-week-old C57BL/6 female mice were used for this experiment. The y-axis is depicted in a log scale. Error bars represent SEM. Student t test was used to compare the groups (P = .0037). (B) Clot weights measured under different diets, as described in Figure 3E, were plotted against values of IDO1 activity measured in the sera of mice described in panel A. A linear correlation between normalized clot weights and IDO activity levels. (C) Representative IF images of IVC from IVC of mice on different Trp diets, stained with antibodies to IDO1 and CD31. DAPI was used for nuclear stain (scale bar, 100 μm). (D) ID of IDO1 labeling normalized to a uniform surface area in all samples. ANOVA (P < .001). Student t test was used to compare groups. (E) Averages of tumor volume in all groups are shown. Error bars represent SEM. (F) Averages of clot weights normalized to the body weight across different groups of mice are shown. Error bars represent SEM. ANOVA was done to compare all groups; P < .0001. Student t test was performed to compare groups. ∗P = .0131; ∗∗P = .0020; ##P = .0011. (G) Average levels of Kyn are shown. Error bars represent SEM. Kyn level measured here under a normal or high Trp diet is somewhat higher than the one recorded in Figure 5C, likely owing to a 3-day longer duration of diet under this experimental condition (see “Results”). The number of mice analyzed under each condition is denoted by dots in each bar graph. Age-matched C57BL/6 female mice were used in all experiments, similar to Figure 1. ANOVA was performed to compare all groups. P = .0012. Student t test was performed to compare groups. #P = .0199; ∗P = .0501; ∗∗P = .0079.
Figure 6.

IDO1 inhibition attenuates thrombosis in a mouse cancer model under different Trp diets. (A) IDO activity measured in the sera of mice with and without MC38 tumor. A group of age-matched 8- to 12-week-old C57BL/6 female mice were used for this experiment. The y-axis is depicted in a log scale. Error bars represent SEM. Student t test was used to compare the groups (P = .0037). (B) Clot weights measured under different diets, as described in Figure 3E, were plotted against values of IDO1 activity measured in the sera of mice described in panel A. A linear correlation between normalized clot weights and IDO activity levels. (C) Representative IF images of IVC from IVC of mice on different Trp diets, stained with antibodies to IDO1 and CD31. DAPI was used for nuclear stain (scale bar, 100 μm). (D) ID of IDO1 labeling normalized to a uniform surface area in all samples. ANOVA (P < .001). Student t test was used to compare groups. (E) Averages of tumor volume in all groups are shown. Error bars represent SEM. (F) Averages of clot weights normalized to the body weight across different groups of mice are shown. Error bars represent SEM. ANOVA was done to compare all groups; P < .0001. Student t test was performed to compare groups. ∗P = .0131; ∗∗P = .0020; ##P = .0011. (G) Average levels of Kyn are shown. Error bars represent SEM. Kyn level measured here under a normal or high Trp diet is somewhat higher than the one recorded in Figure 5C, likely owing to a 3-day longer duration of diet under this experimental condition (see “Results”). The number of mice analyzed under each condition is denoted by dots in each bar graph. Age-matched C57BL/6 female mice were used in all experiments, similar to Figure 1. ANOVA was performed to compare all groups. P = .0012. Student t test was performed to compare groups. #P = .0199; ∗P = .0501; ∗∗P = .0079.

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