Landscape and VAFs of RASp mutations in CMML. (A) Frequency of detected mutations at the time of diagnosis in the cohort of 814 patients with CMML. (B) Heat map detailing the frequency and clonal size of identified RASp mutations among patients with mutations in multiple RAS RASp genes. Each column represents 1 patient. Mutations are color coded based on VAF, and classified among 4 groups: VAF <10%, 10% to 20%, 20% to 40%, and >40%. Asterisks indicate genes for which multiple mutations (∗2 total mutations; ∗∗3 total mutations) were present in each patient. In patients with multiple mutations in each gene, the VAF color code shown corresponds to the mutation with the highest VAF. Cells in gray represent genes that were not sequenced for a specific patient, those in white indicate wildtype state, and cherry color indicates mutation was present but VAF is not available. (C) Violin plots of VAFs of identified RASp mutations among the CMML cohort. Black horizontal lines in violin plots represent the median VAF for a given gene. (D) Violin plots of VAFs of identified RAS pathway mutation among MD-CMML or MP-CMML. Black horizontal lines in violin plots represent the median VAF for a given gene. Asterisks represent statistically significant P values (∗P < .05; ∗∗P < .01). (E) Lollipop plots representing all identified mutations in each of the RASp genes. The height of the bar plot and number within each lollipop bubble represent the frequencies of specific mutations. The color of each gene region represents gene domains.