Deletion of Il6 in MSCs attenuates the OXPHOS status of residual AML cells after Ara-C treatment. (A) Measurement of mitochondrial respiration in AML cells sorted from AML mice using the Seahorse Bioscience XF Analyzer (n = 3). (B) Calculation of basal respiration, maximum respiration, and mitochondrial ATP production based on the measurements in panel A (n = 3). (C-E) FCM analysis of mitochondrial mass (C), membrane potential (D), and mtROS levels (E) in AML cells sorted from AML mice (n = 5). Data are presented as mean ± SD. Differences analyzed using ordinary 1-way ANOVA with Tukey multiple comparisons test (panels C-E) or 2-way ANOVA with Sidak multiple comparisons test (panel B). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. ATP, adenosine triphosphate; Basal, basal respiration; IACS, IACS-010759 (OXPHOS inhibitor); Max, maximal respiration; MFI, mean fluorescence intensity; ns, not significant; OCR, oxygen consumption rate; TMRM, tetramethylrhodamine methyl ester.