Epigenetic landscape and metabolic profile of CGD monocytes. (A) Principal component analysis of the accessible peaks of unstimulated monocytes from HC (n = 5) and CGD (n = 5) detected with ATAC-seq. The first 2 principal components are visualized, where PC1 accounts for 47.976% of the total variance epigenetic profile. (B) Volcano plot of differentially accessible regions mapping in promoter or genic loci of monocytes from HC (n = 5) and patients with CGD (n = 5) assessed by ATAC-seq. Peaks with an adjusted IHW P value <.05 and a mean log₂FC > 1 or < −1 are plotted in dark purple. (C-D) Volcano plots of differentially abundant metabolites between neutrophils (C) and monocytes (D) from HC (n = 3) and patients with CGD (n = 5) assessed by targeted metabolomics. The plots represent log₂FC and −log₁₀ of the corrected P values compared with HC. Metabolites with an adjusted P value < .05 and a mean log₂FC > 1 or < −1 are plotted in dark purple and were considered as differentially regulated. (E-F) Intracellular quantification of triglycerides (F) and free fatty acid (G) in unstimulated monocytes from HC (n = 5) and patients with CGD (n = 4). (E-F) Data are presented as the means ± SEM and statistical analysis was performed using the Mann-Whitney U test between patients and HC; a 2-sided P value <.05 was considered statistically significant. FFA, free fatty acid; IHW, independent hypothesis weighting; kbp, kilobase pairs; ns, not significant; TG, triglyceride; TSS, transcription start site; TTS, transcription termination site.