CGD neutrophils and monocytes present functional metabolic defects. (A) ECAR on Glyco Stress Test in monocytes from HC (n = 13) and CGD (n = 5) and relative metabolic glycolytic parameters. (B) OCR on Mito Stress Test in monocytes from HC (n = 13) and patients with CGD (n = 5) relative metabolic mitochondrial parameters. (C) ECAR on Glyco Stress Test in neutrophils from patients with CGD (n = 5) and HC (n = 3) and relative metabolic glycolytic parameters. (D) Lactate production after 4 hours in unstimulated neutrophils from HC (n = 12) and patients with CGD. (E) Intracellular NADPH concentration (pmol/1 000 000 cells) in monocytes of HC (n = 6) incubated for 6 hours with NADPH oxidase complex inhibitor apocynin (1 mM) or its vehicle and stimulated for zymosan for 2 hours. (F) ECAR and OCR after preincubation of monocytes with apocynin (1 mM) for 6 hours followed by stimulation by zymosan (80 μg/mL) during measurements. Data were corrected for background signal of each condition. In the bar plots of panels A-C, different colors indicate different mutations in patients with CGD as indicated by the panel legends. Data are presented as the means ± SEM. Statistical analysis was performed using the Mann-Whitney U test between patients and HC; a 2-sided P value < .05 was considered statistically significant. DMSO, dimethyl sulfoxide.