Patients with B-ALL display enhancer heterogeneity between individuals. (A) Correlation of accessibility at promoter regions (<2.5 kb from a TSS) measured by ATAC-seq signal between patient with DUX4/ERG, ETV6-RUNX1 (ETV-RUNX), and hyperdiploid subtypes. Data obtained from GSE161501. (B) Correlation of accessibility at putative enhancers (≥2.5 kb from a TSS) as measured by ATAC-seq signal between DUX4/ERG, ETV-RUNX, and hyperdiploid subtypes. (C) Principal component analysis of chromatin accessibility at promoters (left) and enhancers (right) for all 3 B-ALL subgroups. (D) ATAC-seq at the INTS9 locus for hyperdiploid samples. Putative enhancer regions with a high degree of intersample variability are highlighted in blue. (E) ATAC-seq at the SAMD12 locus for ETV6-RUNX1 samples. Putative enhancer regions with a high degree of intersample variability are highlighted in blue. (F) ATAC-seq at the MLLT3 locus for DUX4/ERG samples. Putative enhancer regions with a high degree of intersample variability are highlighted in blue.