Enhancer heterogeneity persists in genetically matched KMT2A::AFF1 leukemias derived from a single donor. (A) Representative flow cytometry plots of the sorting strategy used for KMT2A::AFF1 ALL samples. (B) Scaled H3K27ac level at the 500 most variable enhancer peaks between the 2 KMT2A::AFF1 models. (C) Tornado plot of H3K27ac CUT&Tag signal in 2 HSPC-derived KMT2A::AFF1 ALL models at the 500 most variable enhancer peaks, k-means clustering separates the regions into enhancers showing increased activity in KMT2A::AFF1 ALL 1 (top) vs KMT2A::AFF1 ALL 2 (bottom). (D) CUT&Tag for H3K27ac at the PLXNA4 locus, enhancer regions with increased activity in KMT2A::AFF1 ALL 1 are highlighted in blue. (E) CUT&Tag for H3K27ac at the ENSG00000287092 locus, enhancer regions with increased activity in KMT2A::AFF1 ALL 2 are highlighted in red. FSC, forward scatter; HSPC, hematopoietic stem and progenitor cell.
Figure 3.

Enhancer heterogeneity persists in genetically matched KMT2A::AFF1 leukemias derived from a single donor. (A) Representative flow cytometry plots of the sorting strategy used for KMT2A::AFF1 ALL samples. (B) Scaled H3K27ac level at the 500 most variable enhancer peaks between the 2 KMT2A::AFF1 models. (C) Tornado plot of H3K27ac CUT&Tag signal in 2 HSPC-derived KMT2A::AFF1 ALL models at the 500 most variable enhancer peaks, k-means clustering separates the regions into enhancers showing increased activity in KMT2A::AFF1 ALL 1 (top) vs KMT2A::AFF1 ALL 2 (bottom). (D) CUT&Tag for H3K27ac at the PLXNA4 locus, enhancer regions with increased activity in KMT2A::AFF1 ALL 1 are highlighted in blue. (E) CUT&Tag for H3K27ac at the ENSG00000287092 locus, enhancer regions with increased activity in KMT2A::AFF1 ALL 2 are highlighted in red. FSC, forward scatter; HSPC, hematopoietic stem and progenitor cell.

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