Effects of SEL-based treatment on T-cell marker expression. Violin plots show the comparison between pre- and on-treatment CD3⁺ cells for normalized expression levels of CTLA4 (A), FOXP3 (B), PD-1 (C), LAG3 (D), TIM-3 (E), and VISTA (F). Volcano plots display differential expression for all assessed proteins in CD3⁺ T cells between pretreatment samples and on-treatment samples taken at the time of disease progression (G), or during a period of clinical response (H). FOXP3, forkhead box P3; LAG3, lymphocyte activating 3; PD-1, programmed cell death 1; VISTA, V-set immunoregulatory receptor.