Specific gene mutations are associated with dysplastic features. Forest plots of univariate (open) and multivariable (filled) analyses for gene mutations associated with separated megakaryocyte nuclei (A), abnormal myeloid nuclear segmentation (B), and myeloid hypogranulation (C) in the enrichment cohort. (D-G) Bone marrow biopsy micrographs demonstrate megakaryocytes with widely separated nuclei (arrowheads) in a patient with STAG2, SRSF2, ASXL1, IDH2, KRAS, PTPN11, and subclonal CUX1 and RUNX1 mutations (D) and a patient with germ line GATA2 and secondary somatic STAG2 mutations (E); in contrast, dysplastic megakaryocytes (arrowheads) are present but do not display separated nuclei in a patient with ASXL1, RUNX1, SRSF2, and TET2 mutations (F) or a patient with ASXL1 and SRSF2 mutations (G).