A1 overcomes VEN-acquired resistance in MOLM-13/VEN cells. (A) Growth inhibition of VEN and A1 in MOLM-13/VEN cells treated for 72 hours. (B) MOLM-13 and MOLM-13/VEN cells were treated with 0.8 μM DHA, VEN, DHA + VEN, and A1 for 24 hours. Apoptotic cells were determined by FACS after staining with annexin V/PI (mean ± SD of 3 independent experiments). (C) Volcano plot of the differentially expressed genes in MOLM-13/VEN cells when compared with MOLM-13 cells based on RNA-seq analysis. The red and blue dots represent upregulated and downregulated genes, respectively. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) classification of differentially expressed genes in MOLM-13/VEN cells when compared with MOLM-13 cells. The ordinate shows the KEGG pathway, and the abscissa shows the number of genes under this pathway and its proportion of the total number of genes. (E) Differently expressed apoptosis-related genes between the MOLM-13/VEN cells and the MOLM-13 cells. (F) Differently expressed apoptosis-related proteins in MOLM-13 and MOLM-13/VEN cells as determined by western blot. The right column figure is an image density analysis of 3 independent experiments. (G) Protein regulation of MOLM-13/VEN cells treated with VEN and A1 at the indicated concentrations for 24 hours. The right column figures are image density analyses of 3 independent experiments. ∗P < .05; ∗∗∗P < .001 when compared with the control group. ###P < .001, 2-group comparison. Bim_EL, Bim extralong; Bim_L, Bim long; Bim_S, Bim short; Con, control.