Nuclear height study. Twenty-four hours of 10% stretch induces nuclear deformation. (A) 3-Dimensional (3D) reconstruction of confocal images of HUVEC nuclei in static vs stretched at 10% over a 24-hour period, with Imaris software (left) and model of flattening of the nucleus due to the uniaxial mechanical stretch (right). (B) Frequency distribution of quantification measured by 3D Imaris software, height of the nucleus, volume, projected area of the nucleus, and orientation of the single cell nucleus of the same experiment, N = 1 representative experiment. (C) The bar graphs show the mean and SD of the height and the volume of the cell nucleus for unstretched and stretched conditions 24 hours of 10%. N = 4 independent experiments; n = 4000 cells; ∗∗∗P < .001. deg, degree.
Figure 6.

Nuclear height study. Twenty-four hours of 10% stretch induces nuclear deformation. (A) 3-Dimensional (3D) reconstruction of confocal images of HUVEC nuclei in static vs stretched at 10% over a 24-hour period, with Imaris software (left) and model of flattening of the nucleus due to the uniaxial mechanical stretch (right). (B) Frequency distribution of quantification measured by 3D Imaris software, height of the nucleus, volume, projected area of the nucleus, and orientation of the single cell nucleus of the same experiment, N = 1 representative experiment. (C) The bar graphs show the mean and SD of the height and the volume of the cell nucleus for unstretched and stretched conditions 24 hours of 10%. N = 4 independent experiments; n = 4000 cells; ∗∗∗P < .001. deg, degree.

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