Histone mark acetylation study. Continuous uniaxial cyclic strain for 24 hours induces histone mark acetylation increases. (A) Confocal images of HUVECs: nuclei (DAPI), H3K27ac histone in red, merged colors. Objective, oil original magnification ×40; scale bar, 20 μm. (B) Frequency distribution of static conditions in blue and stretching 10% 24 hours in orange; n = 1 representative experiment. Inset: relative fold change of H3K27ac histone stretched for 24 hours at 10% vs static unstretched state. P < .0001 and mean ± SD values of N = 3 independent experiments; n = 4000 cells. (C) Scatterplot of TM and histone mark intensity signal. (D) Linear relationship between VE cadherins in the nucleus of the cells and histone mark intensity. N = 1 independent experiment; n = 1582 cells. The slope of the linear fit: m = 1.1453; Pearson r coefficient = 0.560.
Figure 7.

Histone mark acetylation study. Continuous uniaxial cyclic strain for 24 hours induces histone mark acetylation increases. (A) Confocal images of HUVECs: nuclei (DAPI), H3K27ac histone in red, merged colors. Objective, oil original magnification ×40; scale bar, 20 μm. (B) Frequency distribution of static conditions in blue and stretching 10% 24 hours in orange; n = 1 representative experiment. Inset: relative fold change of H3K27ac histone stretched for 24 hours at 10% vs static unstretched state. P < .0001 and mean ± SD values of N = 3 independent experiments; n = 4000 cells. (C) Scatterplot of TM and histone mark intensity signal. (D) Linear relationship between VE cadherins in the nucleus of the cells and histone mark intensity. N = 1 independent experiment; n = 1582 cells. The slope of the linear fit: m = 1.1453; Pearson r coefficient = 0.560.

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