HFD delays fibrinolysis and exacerbates the DVT load in mice. (A) Schematic of the experimental model. The mice were fed an ND or an HFD for 4 months, after which tail bleeding assays were performed. After 2 weeks of recovery, each group of mice was randomly selected to evaluate the DVT burden. The remaining mice were euthanized, and sodium citrate anticoagulated blood and tissues were collected (N = 9). (B) Mean body weight (N = 9). (C) Plasma triglyceride and cholesterol levels (N = 9). (D-E) Tail bleeding time (N = 9). (F) Thrombus weight and length (N = 5). (G) Plasma FDP levels (N = 4). (H) Representative curves for plasma fibrin formation and lysis tests and parameters of the curves for fibrinolysis (N = 4). The data are represented as the mean ± standard error of the mean (SEM), n.s. P > .05; ∗P < .05; ∗∗P < .01; ∗∗∗P < .001 vs ND group. BT, bleeding time; n.s., no significance.