Prediction of the upstream regulator of PAI-1 in obesity. (A) UMAP plots of cell populations in the human liver dataset (GSE192740). (B) Expression of Serpine1 across different cell types in the human liver dataset (GSE192740). (C) KEGG pathway analysis of differentially expressed liver genes in human livers (GSE192740). (D) FXR related genes in the BA pathway in hepatocytes (GSE192740). (E) KEGG analysis of liver differential genes in obese and normal weight individuals (GSE130991). (F) KEGG analysis of liver differential genes in baboons before and after high-fat feeding in GSE139981. (G) Heat map showing expression of PAI-1 (Serpine1), FXR (NR1H4), and FXR target genes (NR0B2 and ABCB11) in human livers from 737 non-statin-treated patients, stratified by BMI (GSE130991). (H) The expression of PAI-1, FXR and FXR direct targets in baboon liver samples (GSE139981) at 0 weeks, 7 weeks and 2 years post HFD. (I-K) Correlation coefficients of PAI-1 with FXR and FXR direct target genes in baboon livers (GSE139981). UMAP, uniform manifold approximation and projection.