In vitro differentiated γδ T cells from MND-c.o.RAG1-rescued RAG1-SCID patient HSPCs have more immature TRD D-D rearrangements remaining compared to healthy controls. GeneScan visualization of all Vγ-Jγ rearrangements of the TRG (left column). Right column shows different Dδ2-Jδ, Dδ2-Dδ3, Vδ-Jδ and Vδ-Dδ3 rearrangements of TRD. Jδ is labeled blue and Dδ3 is green. (A) TRG and TRD rearrangements in healthy control PBMCs (used as polyclonal assay control), ATOs differentiated from mPB and UCB CD34⁺ HSPCs and a clonal assay control (cell line⁴⁰) for comparison. (B) ATOs with differentiated patients’ CD34⁺ HSPCs, corrected (+LV) and noncorrected.