BM CD8⁺ T cells exhibit impaired proliferation, elevated BNIP3, and decreased c-Myc and Rheb expression in MM. Paired BM and peripheral blood (PB) mononuclear cells from patients with newly diagnosed MM were analyzed by flow cytometry for: (A) frequency of CD8⁺ T cells; (B) frequency of naïve, CM, EM, and EMRA populations within total CD8⁺ T cells; (C-D) frequency of Ki67-expressing cells within (C) total CD8⁺ T cells and (D) indicated populations, (E-G) BNIP3 expression within (E) total indicated populations and (F-G) Ki67⁺ and Ki67⁻ cells, (F) representative histograms of total CD8⁺ T cells, and (G) fold change of BNIP3 abundance in Ki67⁺ vs Ki67⁻ cells of indicated populations, (H) Rheb abundance (representative histograms of total CD8⁺ T cells and mean within indicated populations), and (I) c-Myc abundance (representative histograms of total CD8⁺ T cells and mean within indicated populations). For all analyses n = 8 independent paired samples, data are shown as (B-D) percentage of parent population, or (E-I) MFI. Where normalized (H), this is to the average (mean) MFI of all populations from matched samples for each individual patient. P values were calculated by (C,G) paired t test, and (D, H, I) 2-way ANOVA and Holm-Sidak’s post hoc test. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001.