FlowCT analysis of BD OneFlow PCD tube. (A) The scheme illustrates the experimental design. (B) UMAP of eosinophils (CD45^+bright, SCC^high), erythroblasts (CD45⁻, CD38⁻, SCC^low), granulocytes (CD45^+dim, SCC^high), lymphocytes (CD45⁺, SCC^low), monocytes (CD45⁺, SCC^int), and plasma cells (CD138⁺ CD38^+high) identified by self-organizing map (SOM) of BD OneFlow PCD tube. (C) Box plots show a statistically different distribution of lymphocytes, GLR, GTL ratio, erythroblasts, monocytes, and monocyte-to-lymphocyte ratio (MLR) as MM progresses. (D) Subclustering of lymphocyte subsets based on the median expression of the individual marker in each cell cluster. Box plots represent the proportion of immature B cells (CD38⁺ CD28⁻ CD27⁻ CD81⁺, CD117⁻) between patients with MGUS, SMM, and MM. (E) Subclustering of monocytes cell subsets based on the median expression of the individual marker in each cell cluster. UMAP, uniform manifold approximation and projection.