FlowCT analysis of BD OneFlow PCST tube. (A) UMAP of eosinophils (CD45^+bright, SCC^high), erythroblasts (CD45⁻, CD38⁻, SCC^low), granulocytes (CD45^+dim, SCC^high), T lymphocytes (CD19⁻, CD45⁺, CD56⁻, CD38^−/+, SCC^low), B lymphocytes (CD19⁺, CD45⁺, SCC^low), NK cells (CD19⁻, CD45⁺, CD56^+dim/+bright, SCC^low), and plasma cells (CD138⁺ CD38^+high) identified by SOM of BD OneFlow PCST tube. (B) Box plots show the statistically different distribution of lymphocytes, T lymphocytes, NK cells, GLR, GTL ratio, MLR, monocytes, and erythroblasts as MM progresses. (C) Subclustering of lymphocyte subsets based on the median expression of the individual marker in each cell cluster. Box plots represent the proportion of NK cells CD38⁺ CD56^dim and B lymphocytes CD38⁺ κ⁻ γ⁻ between patients with MGUS, SMM, and MM. (D) Subclustering of monocyte subsets based on the median expression of the individual marker in each cell cluster. UMAP, uniform manifold approximation and projection.