Granulocytes derived from the BM potentiate the cytotoxicity mediated by Dara. (A) Box plots depict the percentage of viable MM cells (7-amino-actinomycin D negative and Annexin-V negative) from the AMO-1 MM cell line after coculture with peripheral blood neutrophils and PBMCs in the presence of Dara. (B) Schematic diagram of the experimental setup for assessing cytotoxicity mediated by autologous BM-derived granulocytes. (C) Box plot showed the differences in the number of primary autologous MM cells after coculture with BM-derived granulocytes and BMMCs from patients with MM. Primary autologous MM cell counts were normalized by comparing the absolute number of viable MM cells in Dara-treated vs untreated conditions. Abs. no., absolute number; Dara, daratumumab.