Western blot analysis of HO-1 expression in the liver of C57Bl/6J mice transduced with 1 × 10¹⁴ vg/kg rAAV8-hHPX or rAAV8-mHPX vector and challenged with 70 μM/kg of heme. Samples were obtained 1 hour after infusion. (A) Lower protein expression is demonstrated in gels and expressed as AUs (B) after densitometry.