HbF induction in HBG1 promoter edited animals. (A-B) Flow cytometric analysis of the percentage of RBCs containing HbF (F+) in adult injected (A) and neonatally injected animals (B) at the terminal time point. (C-D) HPLC analysis of percent HbF in blood lysates collected at terminal time point from adult injected (C) and neonatally injected animals (D). (E-F) CBC analysis at the terminal time point for hemoglobin levels (gram/deciliter) in adult injected (E) or neonatally injected animals (F). (G-H) CBC analysis at the terminal time point for RBC count (×10⁶ cells per μL) in adult injected (G) or neonatally injected mice (H). One-way ANOVA with Brown-Forsythe and Welch correction. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. Data compiled from 4 (panels A,C,E) or 3 (panels B,D,F) independent experiments. Females, square; males, triangles. A/A, healthy HBB^A/A; S/S, sickle homozygote HBB^S/S.